Facsaria manual

BD FACSAria I Operation Manual – Fluidics Startup Shutdown and CST 2 5. Check that the ND filter (next to flow cell) is ; and close the flow cell access door. 6. Click ‘Run’ to initiate the performance check, and then ‘OK’ to confirm. User MAY select ‘Abort’ to stop the test and cancel the CST Run. 7. FACSAria II Sample Preparation Acceptable sample tubes are polypropylene 12 × 75 mm, mL tubes, bullet tubes, or mL microcentrifuge tubes. Concentration should be 10–20 × 10 6 cells per mL (allows for ideal pressure and best use of time). FACSAria™ cell sorter portfolio. Since its introduction in , each successive generation of the BD FACSAria has opened the complex world of cell sorting to a broader audience of researchers and wider range of applications. Now, the BD FACSAria III system .

The BD FACSAria™ III Sorter has been used in sorting Treg cells. Representative FoxP3 staining of sorted Tregs. To determine purity, as defined by FoxP3+ status, a portion of the cells was stained with an anti-human FoxP3 antibody conjugated to the BD Horizon™ V Dye. Data is representative of 10 experiments. BD FACSAria I Operation Manual 10 FSC (correspond to the Cell Size) and SSC (correspond to the Cell Granularity) are MUST for all kind of analysis. Both FSC and SSC ought to be displayed in linear scale, hence their ‘L’ boxes should not be ticked. Unless assigned for cell cycle and/or DNA analysis, fluorescence channels must be checked. The new BD FACSAria™ II cell sorter improves ease of use, flexibility, and aseptic capability to make it the best choice for consistent results in sorting across a broad range of applications. The heart of this cell sorting revolution is the true fixed-alignment flow cell, gel-coupled to the collection optics.

7 de ago. de User's Guide for FACS Aria III. Important notes. • Always filter samples before loading them into the sorter! Core Facilities Core Customer Help Manual. WHAT YOU NEED TO BRING. Cells should be at a concentration of 5x to 10x/ml; Samples. Faculty Core Facility, Li Ka Shing Faculty of Medicine. BD FACSAria SORP Operation Manual. 1. FACSAria SORP Standard Operation Protocol. Basic Operation.