Facs manual compensation

Fluorescence compensation. Emission spectra of two fluorophores commonly used in flow cytometry, FITC and PE are shown. Also shown is a graphical representation of two commonly used filters, /50 and /40, to detect these fluorophores. Shown in red is the portion of the FITC spectrum that will be detected in the PE detector (/40) and. VenturiOne – Manual Compensation in Flow Cytometry. VenturiOne is our rapid data analysis platform to process, interpret, and report on your FCS data files – using features such as manual compensation to interpret your flow cytometry data. In our VenturiOne tutorial series, you can find out how to process multiple files, use our compensation wizard, autogating and more on VenturiOne. Compensation is correctly set when the median of the negative population is equal to the median of the positive population in the spillover channel. For example, if using a positive FITC-stained sample to apply compensation to the PE channel, the median of the negative and the FITC-positive populations should be equal in the PE channel.

Select Experiment Compensation Setup Calculate Compensation. When the Single Stained Setup box appears, select “Link Save” to apply the calculated values to your experiment. Load a tube of water on to the instrument and place it in STANDBY. Return to your original sample tube (“Tube_”) in the Browser. Compensation is correctly set when the median of the negative population is equal to the median of the positive population in the spillover channel. For example, if using a positive FITC-stained sample to apply compensation to the PE channel, the median of the negative and the FITC-positive populations should be equal in the PE channel. Learn principles of compensation for your Flow Cytometry data analysis. By the end of this tutorial, you should be able to understand: What is compensation?.

This bimodal distribution can be used for single-color compensation controls in multicolor flow cytometry experiments. OneComp eBeads® are designed for use in. BD FACS lysing solution: US 4,,; 4,,; 5,, Recording compensation controls. If you choose to perform compensation manually. During the early years of multicolor flow cytometry, where most researchers were using fluorochromes, manual compensation was the vogue method to.